S100A12 蛋白
Quick Overview for S100A12 蛋白 (ABIN7319473)
抗原
See all S100A12 蛋白蛋白类型
宿主
资源
纯度
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原理
- Recombinant Human S100A12/CAGC Protein
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序列
- Met1-Glu92
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产品特性
- Recombinant Human S100A12 is produced by our E.coli expression system and the target gene encoding Met1-Glu92 is expressed.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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S100 Calcium Binding Protein A12 (S100A12) (AA 2-92) protein (His-IF2DI Tag)
S100A12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
S100 Calcium Binding Protein A12 (S100A12) (AA 1-92) protein (GST tag)S100A12 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- S100A12 (S100 Calcium Binding Protein A12 (S100A12))
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别名
- S100A12/CAGC
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背景
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Background: There are at least 21 different S100 proteins and the protein is 100 % soluble in ammonium sulfate at neutral pH . S100 proteins play a role in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A12 is characterized by two EF-hand calcium-binding motifs, zinc- and copper-binding protein.S100A12 is a disulfide-linked homodimer and the interface between the two subunits is composed mostly of hydrophobic residues.Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. EN-RAGE acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. It also acts as a monocyte and mast cell chemoattractant. Moreover, it can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. It can inhibit the activity of matrix metalloproteinases, MMP2, MMP3 and MMP9 by chelating Zn2+ from their active sites.
Synonym: Protein S100-A12,Calcium-binding protein in amniotic fluid 1,Calgranulin-C,Extracellular newly identified RAGE-binding protein,Migration inhibitory factor-related protein 6,S100 calcium-binding protein A12,Calcitermin,S100A12,CGRP,MRP-6,EN-RAGE
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分子量
- 10.6 kDa
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UniProt
- P80511
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途径
- Toll-Like Receptors Cascades, S100 Proteins
抗原
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