ERBB3 Protein (AA 730-1065) (His-GST)
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Quick Overview for ERBB3 Protein (AA 730-1065) (His-GST) (ABIN7197657)
抗原
See all ERBB3 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 730-1065
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标记
- This ERBB3 protein is labelled with His-GST.
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原理
- Recombinant Human HER3/ErbB3 Protein (aa 730-1065, His &GST Tag)
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序列
- Pro 730-Ser 1065
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产品特性
- A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. No Kinase Activity 2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST bound to human NRG1 (aa 2-224) was 20 nM.
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Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 20-643) protein (His-Avi Tag)
ERBB3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 20-643) protein (His tag)ERBB3 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 20-643) (Active) protein (Biotin,His-Avi Tag)ERBB3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 20-643) (Active) protein (hFc Tag)ERBB3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 20-641) (Active) protein (His tag)ERBB3 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 21-331) protein (GST tag)ERBB3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 1005-1230) protein (His tag)ERBB3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 544-643) protein (mFc Tag)ERBB3 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 544-643) protein (Fc Tag)ERBB3 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 20-643) protein (hIgG-His-tag)ERBB3 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.5, 10 % glycerol, 1 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
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别名
- HER3/ErbB3
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背景
- EEBB3,ErbB-3,HER3,LCCS2,MDA-BF-1,c-erbB-3,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3,ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
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分子量
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Calculated MW: 65.0 kDa
Observed MW: 65 kDa
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基因ID
- 2065
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NCBI登录号
- NP_001973
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UniProt
- P21860
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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