NOG Protein (AA 28-232)
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北京 101111
Quick Overview for NOG Protein (AA 28-232) (ABIN7319511)
抗原
See all NOG 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 28-232
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原理
- Recombinant Human Noggin/NOG Protein
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序列
- Gln28-Cys232
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产品特性
- Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 0.01 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 10-100ng/mL in the presence of 200 ng/mL of Recombinant Human BMP-4(#CR93).
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Noggin (NOG) (AA 20-232) (Active) protein
NOG 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) (AA 28-232) (Active) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Noggin (NOG) (AA 28-232) protein (His-IF2DI Tag)NOG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Noggin (NOG) (AA 28-232) protein (GST tag)NOG 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Noggin (NOG) (AA 28-232) protein (His tag)NOG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. CC
Noggin (NOG) (AA 28-232) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- NOG (Noggin (NOG))
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别名
- Noggin/NOG
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背景
- NOG,Noggin,Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99 % , 99 %, 98 %, 97 % and 89 % aa sequence identity with mouse, rat bovine, equine and chicken Noggin, respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.
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分子量
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Calculated MW: 23 kDa
Observed MW: 25-33 kDa
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基因ID
- 9241
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UniProt
- Q13253
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途径
- Stem Cell Maintenance, Tube Formation
抗原
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