NOG Protein (His tag)
Quick Overview for NOG Protein (His tag) (ABIN7197212)
抗原
See all NOG 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This NOG protein is labelled with His tag.
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原理
- Recombinant Human Noggin/NOG Protein (His Tag)(Active)
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序列
- Met 1-Cys 232
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产品特性
- A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with a polyhistidine tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.05-0.3 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
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Noggin (NOG) (AA 20-232) (Active) protein
NOG 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) (AA 28-232) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Noggin (NOG) (AA 28-232) (Active) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) protein (His tag)NOG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) (AA 28-232) protein (His tag)NOG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Noggin (NOG) (AA 28-232) protein (GST tag)NOG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Noggin (NOG) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) (AA 28-232) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- NOG (Noggin (NOG))
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别名
- Noggin/NOG
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背景
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Background: Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.
Synonym: Noggin;SYM1;SYNS1
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分子量
- 24.6 kDa
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NCBI登录号
- NP_005441
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途径
- Stem Cell Maintenance, Tube Formation
抗原
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