IL2 Receptor beta 蛋白
Quick Overview for IL2 Receptor beta 蛋白 (ABIN7196471)
抗原
See all IL2 Receptor beta (IL2RB) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Recombinant Human CD122/IL-2RB Protein (Active)
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序列
- Met 1-Asp 239
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产品特性
- The mature form of human IL2 Rβ (NP_000869.1) extracellular domain (Met 1-Asp 239) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- 1. Measured by its binding ability in a functional ELISA. Immobilized human IL2Rb at 10 μg/ml (100 μl/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 μg/mL.2. Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1-4μg/mL.
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Interleukin 2 Receptor, beta (IL2RB) (AA 27-239) protein (His-Avi Tag)
IL2RB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, beta (IL2RB) (AA 27-239) protein (Fc Tag)IL2RB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, beta (IL2RB) (AA 27-239) protein (His-Avi Tag,Biotin)IL2RB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, beta (IL2RB) (AA 27-239) protein (His tag)IL2RB 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, beta (IL2RB) (AA 27-239) protein (His tag,Biotin)IL2RB 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by Tris-Bis PAGE, >90 % as determined by HPLC
Interleukin 2 Receptor, beta (IL2RB) (AA 27-239) (Active) protein (Fc Tag)IL2RB 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, beta (IL2RB) protein (Fc Tag)IL2RB 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 2 Receptor, beta (IL2RB) protein (His tag)IL2RB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 2 Receptor, beta (IL2RB) (AA 27-239) protein (His tag)IL2RB 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 100 mM NaCl, 50 mM Tris, pH 7.5
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))
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别名
- CD122/IL-2RB
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背景
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Background: Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-, prepro B cells, fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.
Synonym: CD122,IL15RB,P70-75
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分子量
- 25.2 kDa
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NCBI登录号
- NP_000869
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途径
- JAK/STAT Signaling, Growth Factor Binding
抗原
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