IL-10 Protein (AA 19-178)
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北京 101111
Quick Overview for IL-10 Protein (AA 19-178) (ABIN7318100)
抗原
See all IL-10 (IL10) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 19-178
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原理
- Recombinant Human IL-10/Interleukin-10 Protein
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序列
- Ser19-Asn178
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产品特性
- Recombinant Human Interleukin-10 is produced by our Mammalian expression system and the target gene encoding Ser19-Asn178 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 0.01 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Loaded Human IL-10RA-His(PKSH033632) on HIS1K Biosensor, can bind Human IL-10 (PKSH032005) with an affinity constant of 1 pM as determined in BLI assay.
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Interleukin 10 (IL10) (AA 19-178) (Active) protein (Biotin,His-Avi Tag)
IL10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 10 (IL10) (AA 19-178) (Active) protein (His-Avi Tag)IL10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 10 (IL10) (AA 19-178) (Active) proteinIL10 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 10 (IL10) (AA 1-178) protein (GST tag)IL10 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 10 (IL10) (Active) proteinIL10 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 10 (IL10) (AA 19-178) protein (His tag)IL10 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Interleukin 10 (IL10) (AA 22-178) protein (His tag)IL10 宿主: Hamster 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 10 (IL10) (AA 19-178) proteinIL10 宿主: 人 宿主: Mammalian Cells Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 10 (IL10) (AA 19-178) (Active) protein (His tag)IL10 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 10 (IL10) (AA 19-178) (Active) protein (His tag)IL10 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- IL-10/Interleukin-10
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背景
- Interleukin,RP11-262N,CSIF,GVHDS,IL-10,IL10A,TGIF,IL10,Cytokine synthesis inhibitory factor,Interleukin-10,MGC126450,MGC126451,RP11-262N9.1,Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF),is a secreted protein and belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in produced in several T helper populations, it is proposed that it provides a feedback loop to limit the effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations.
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分子量
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Calculated MW: 18.6 kDa
Observed MW: 16 kDa
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基因ID
- 3586
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UniProt
- P22301
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
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