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Quick Overview for ICAM2 蛋白 (ABIN7196247)
抗原
See all ICAM2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Recombinant Mouse ICAM-2/CD102 Protein
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序列
- Met 1-Gln 222
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产品特性
- A DNA sequence encoding the mouse ICAM2 (NP_034624.1) (Met 1-Gln 222)was expressed with six amino acids (LEVLFQ) at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rmICAM1 coated plates (12.5 μg/mL with 100 μL/well), > 30% cells will adhere after 1 hour incubation at 37°C.
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Intercellular Adhesion Molecule 2 (ICAM2) (AA 22-223) protein (His tag)
ICAM2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Intercellular Adhesion Molecule 2 (ICAM2) (AA 25-223) protein (His tag)ICAM2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Intercellular Adhesion Molecule 2 (ICAM2) (Active) protein (Fc Tag)ICAM2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 93 % as determined by reducing SDS-PAGE. CC Active
Intercellular Adhesion Molecule 2 (ICAM2) (AA 1-275) protein (GST tag)ICAM2 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Intercellular Adhesion Molecule 2 (ICAM2) (AA 23-222) protein (Fc Tag)ICAM2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))
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别名
- ICAM-2/CD102
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背景
- AILIM Protein,CCLP Protein,CRP-1 Protein,H4 Protein,Ly115 Protein,Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.
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分子量
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Calculated MW: 23.6 kDa
Observed MW: 38-42 kDa
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基因ID
- 15896
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NCBI登录号
- NP_034624
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UniProt
- P35330
抗原
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