GADD45A Protein (His-GST)
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Quick Overview for GADD45A Protein (His-GST) (ABIN7317638)
抗原
See all GADD45A 蛋白蛋白类型
宿主
资源
纯度
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标记
- This GADD45A protein is labelled with His-GST.
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原理
- Recombinant Human GADD45A/DDIT-1 Protein (His & GST Tag)
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序列
- Met 1-Arg 165
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产品特性
- A DNA sequence encoding the full length of human GADD45A (P24522) (Met 1-Arg 165) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 116,946.14 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (His tag)
custom-made GADD45A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (His tag)custom-made GADD45A 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (GST tag)GADD45A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) protein (Myc-DYKDDDDK Tag)GADD45A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
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别名
- GADD45A/DDIT-1
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背景
- DDIT-1,DDIT1,DNA Damage-Inducible Transcript 1 Protein,GADD45,GADD45A,Growth Arrest and DNA Damage-Inducible Protein GADD45 Alpha,GADD45A is a member of the GADD45 Family, and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions, including MAPK signaling, cell cycle regulation, DNA repair and genomic stability, apoptosis, and immune responses. The GADD45 Family of genes is rapidly induced by different stressors, including differentiation-inducing cytokines, and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins, including Cdc2 protein kinase, proliferating cell nuclear antigen, p21Waf1/Cip1 protein, core histone protein and MTK/MEKK4, an up-stream activator of the JNK/SAPK pathway, indicating that GADD45A may play important roles in the control of cell cycle checkpoint, DNA repair process, and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario, wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues, p63 and p73. The decreased GADD45A expression is also considered a survival mechanism, as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells, it presents itself as an emerging drug target worthy of further investigation.
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分子量
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Calculated MW: 46.2 kDa
Observed MW: 46 kDa
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基因ID
- 1647
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UniProt
- P24522
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途径
- p53 Pathway, Cell Division Cycle
抗原
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