Quick Overview for FCGR2A Protein (His tag) (ABIN7195679)
抗原
See all FCGR2A 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This FCGR2A protein is labelled with His tag.
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原理
- Recombinant Human CD32a/FCGR2A Protein (167 His, His Tag)(Active)
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序列
- Met 1-Ile 218
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产品特性
- A DNA sequence encoding (Met 1-Ile 218) of human CD32a (NP_001129691.1) was expressed with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized human CD32a-His (R131H) (CHO) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.09-0.21 μg/ml.
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Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 36-218) protein (His-Avi Tag)
FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 28-208) protein (His tag)FCGR2A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 36-218) protein (His-Avi Tag,Biotin)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 36-218) protein (His-Avi Tag,Biotin)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SEC-HPLC, SPR
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 46-150) protein (GST tag)FCGR2A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) protein (Fc Tag)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (Active) protein (Fc Tag)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-216) protein (His tag)FCGR2A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (Active) protein (Biotin,His-Avi Tag)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) proteinFCGR2A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
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别名
- CD32a/FCGR2A
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背景
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Background: Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
Synonym: Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, FCG2, FCGR2A1,IGFR2,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2
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分子量
- 22 kDa
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NCBI登录号
- NP_001129691
抗原
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