EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST)
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Quick Overview for EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST) (ABIN7317076)
抗原
See all EPH Receptor A4 (EPHA4) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 570-986
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标记
- This EPH Receptor A4 protein is labelled with His-GST.
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原理
- Recombinant Human EphA4 Protein (aa 570-986, His &GST Tag)
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序列
- Ser 570-Val 986
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产品特性
- A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized human EPHA4 (aa 570-986)at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.
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EPH Receptor A4 (EPHA4) (AA 20-547) (Active) protein (His tag)
EPHA4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
EPH Receptor A4 (EPHA4) (AA 1-986) protein (GST tag)EPHA4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 887-986) protein (GST tag)EPHA4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 1-105) protein (GST tag)EPHA4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 20-547) protein (His tag)EPHA4 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
EPH Receptor A4 (EPHA4) (AA 20-547) protein (His tag)EPHA4 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % SDS
EPH Receptor A4 (EPHA4) (AA 1-547) (Active) protein (His tag)EPHA4 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by reducing SDS-PAGE. ELISA Active
EPH Receptor A4 (EPHA4) (AA 1-547) (Active) protein (Fc Tag)EPHA4 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by reducing SDS-PAGE. ELISA Active
EPH Receptor A4 (EPHA4) (AA 20-547) (Active) protein (His tag)EPHA4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10 % glycerol, 3 mM DTT
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- EPH Receptor A4 (EPHA4)
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别名
- EphA4
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背景
- EK8;EPH-like kinase 8;EPHA4;Ephrin type-A receptor 4;HEK8;SEK;TYRO1;Tyrosine-protein kinase TYRO1;Tyrosine-protein kinase receptor SEK;hEK8,EPH receptor A4 (ephrin type-A receptor 4); also known as EphA4; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus; and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling; which is triggered by ephrins7; probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands; which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.
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分子量
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Calculated MW: 75.0 kDa
Observed MW: 67 kDa
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基因ID
- 2043
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UniProt
- P54764
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途径
- RTK signaling
抗原
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