CDK1 Protein (AA 1-297) (His-GST)
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Quick Overview for CDK1 Protein (AA 1-297) (His-GST) (ABIN7195307)
抗原
See all CDK1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 1-297
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标记
- This CDK1 protein is labelled with His-GST.
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原理
- Recombinant Mouse CDC2 Kinase/CDK1 Protein (His & GST Tag)
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序列
- Met1-Met297
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产品特性
- A DNA sequence encoding the mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Kinase activity untested
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Mouse价格从 116,946.14 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Mouse价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) protein (His tag)
custom-made CDK1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) protein (His tag)custom-made CDK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) protein (GST tag)CDK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) protein (His tag)CDK1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % SDS
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
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别名
- CDC2 Kinase/CDK1
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背景
- CDC2>Cdc2;Cdc2a;p34<,CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
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分子量
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Calculated MW: 61.9 kDa
Observed MW: 57 kDa
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UniProt
- P11440
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
抗原
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