Cyclin E1 Protein (CCNE1) (AA 1-408) (His-GST)
This Recombinant Cyclin E1 protein is expressed in Baculovirus infected Insect Cells.
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产品编号 ABIN7320118
发货至:
中国
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Quick Overview for Cyclin E1 Protein (CCNE1) (AA 1-408) (His-GST) (ABIN7320118)
抗原
See all Cyclin E1 (CCNE1) 蛋白
Cyclin E1 (CCNE1)
蛋白类型
Recombinant
宿主
小鼠
资源
Baculovirus infected Insect Cells
纯度
> 90 % as determined by reducing SDS-PAGE.
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产品特性
- AA 1-408
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标记
- This Cyclin E1 protein is labelled with His-GST.
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原理
- Recombinant Mouse CCNE1/Cyclin-E1 Protein (His & GST Tag)
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序列
- Met1-Glu408
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产品特性
- A DNA sequence encoding the mouse CCNE1 (AAH62152.1) (Met1-Glu408) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Cyclin E1 (CCNE1) (AA 1-410) protein (GST tag)
CCNE1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM Tris, 100 mM Nacl, 3 mM DTT, 0.5 mM GSH, 10 % glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Cyclin E1 (CCNE1)
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别名
- CCNE1/Cyclin-E1
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背景
- AW538188,CycE1,Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.
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分子量
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Calculated MW: 74.8 kDa
Observed MW: 75 kDa
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UniProt
- Q61457
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途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
抗原
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