Cyclin E1 Protein (CCNE1) (His-GST)
This Recombinant Cyclin E1 protein is expressed in Baculovirus infected Insect Cells.
产品编号 ABIN7317726
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Cyclin E1 Protein (CCNE1) (His-GST) (ABIN7317726)
抗原
See all Cyclin E1 (CCNE1) 蛋白
Cyclin E1 (CCNE1)
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
Baculovirus infected Insect Cells
应用范围
ELISA
纯度
> 82 % as determined by reducing SDS-PAGE.
-
-
标记
- This Cyclin E1 protein is labelled with His-GST.
-
原理
- Recombinant Human CCNE1/Cyclin-E1 Protein (His & GST Tag)
-
序列
- Met 1-Ala 410
-
产品特性
- A DNA sequence encoding the full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Immobilized human HG3C-CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.
-
-
想要此Protein的其他选项吗？
!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
Cyclin E1 (CCNE1) (AA 1-410) protein (GST tag)
CCNE1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Cyclin E1 (CCNE1) (AA 1-408) protein (His-GST)CCNE1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, 1 mM GSH, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- Cyclin E1 (CCNE1)
-
别名
- CCNE1/Cyclin-E1
-
背景
- CCNE,Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.
-
分子量
-
Calculated MW: 75.0 kDa
Observed MW: 70 kDa
-
基因ID
- 898
-
NCBI登录号
- NP_001229
-
UniProt
- P24864
-
途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
抗原
-
You are here:
