Chitotriosidase 1 Protein (His tag)
This Recombinant Chitotriosidase 1 protein is expressed in HEK-293 Cells.
产品编号 ABIN7195100
发货至:
中国
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Quick Overview for Chitotriosidase 1 Protein (His tag) (ABIN7195100)
抗原
See all Chitotriosidase 1 (CHIT1) 蛋白
Chitotriosidase 1 (CHIT1)
(Chitotriosidase (CHIT1))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 94 % as determined by reducing SDS-PAGE.
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标记
- This Chitotriosidase 1 protein is labelled with His tag.
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原理
- Recombinant Human Chitotriosidase/CHIT1 Protein (His Tag)
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序列
- Met 1-Asn 466
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产品特性
- A DNA sequence encoding the human CHIT1 (NP_003456.1) (Met 1-Asn 466) precursor was fused with a polyhistidine tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Chitotriosidase (CHIT1) (AA 1-466) protein (GST tag)
CHIT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chitotriosidase (CHIT1) protein (Myc-DYKDDDDK Tag)CHIT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 12.5 mM Tris, 75 mM NaCl, 50 % glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
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别名
- Chitotriosidase/CHIT1
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背景
- CHI3,CHIT,CHIT1,CHITD,Chitinase-1,Chitotriosidase-1,Chitotriosidase, also known as Chitinase-1 and CHIT1, is a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily. It is a member of the mammalian chitinase family, structurally homologous to chitinases from other species, is synthesized and secreted by specifically activated macrophages. Chitotriosidase is a polymer of N-acetylglucosamine. Serum and plasma chitotriosidase activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase, its increased expression was noted in several lysosomal storage diseases and atherosclerosis. In addition to lipid storage disorders, where Chit activity has longer been used as a marker of disease activity and therapeutic response, elevation of plasma Chit may occur in hematological disorders with storage of erythrocyte membrane breakdown products as thalassemia and different systemic infectious diseases sustained by fungi and other pathogens. Recently, increased Chit activity was demonstrated in CNS from patients with different neurological disorders. Chitotriosidase is believed to play a role in mechanisms of immunity and protection against chitin-containing pathogens.
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分子量
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Calculated MW: 50.8 kDa
Observed MW: 48 kDa
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基因ID
- 1118
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NCBI登录号
- NP_003456
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UniProt
- Q13231
抗原
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