CD36 Protein (CD36) (His-Fc tag)
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Quick Overview for CD36 Protein (CD36) (His-Fc tag) (ABIN7320283)
抗原
See all CD36 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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标记
- This CD36 protein is labelled with His-Fc tag.
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原理
- Recombinant Mouse CD36/SCARB3 Protein (His & Fc Tag)
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序列
- Gly 30-Lys 439
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产品特性
- A DNA sequence encoding the extracellular domain of mouse CD36 (NP_001153030.1) (Gly 30-Lys 439) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 Fc chimera with a linear ranger of 6. 4-800 ng/ml.
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CD36 (CD36) (AA 30-439) protein (Fc Tag)
CD36 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD36 (CD36) protein (DYKDDDDK Tag)CD36 宿主: 人 宿主: HEK-293 Cells Synthetic Nanodisc ELISA, Imm, SPR, PhD, cryo-EM
CD36 (CD36) (AA 30-439) (Active) protein (His tag)CD36 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD36 (CD36) (AA 30-439) (Active) protein (Biotin,His-Avi Tag)CD36 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 85 % as determined by HPLC Active
CD36 (CD36) (AA 30-439) (Active) protein (His-Avi Tag)CD36 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
CD36 (CD36) (AA 30-439) (Active) protein (His tag)CD36 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
CD36 (CD36) (AA 42-178) protein (His-IF2DI Tag)CD36 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
CD36 (CD36) (AA 30-439) protein (Fc Tag)CD36 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by reducing SDS-PAGE.
CD36 (CD36) (Active) protein (His tag)CD36 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by reducing SDS-PAGE. ELISA Active
CD36 (CD36) (AA 30-439) protein (His tag)CD36 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD36
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别名
- CD36/SCARB3
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背景
- FAT,GPIV,Scarb3,The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell types in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.
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分子量
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Calculated MW: 74.5 kDa
Observed MW: 110-120 kDa
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基因ID
- 12491
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NCBI登录号
- NP_001153030
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UniProt
- Q08857
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途径
- TLR signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins
抗原
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