PD-L1 Protein (DYKDDDDK Tag)
Our Local Distributor
北京 101111
Quick Overview for PD-L1 Protein (DYKDDDDK Tag) (ABIN7318878)
抗原
See all PD-L1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
标记
- This PD-L1 protein is labelled with DYKDDDDK Tag.
-
原理
- Recombinant Human PD-L1/B7-H1/CD274 Protein (Flag Tag)
-
序列
- Phe19-Thr 239
-
产品特性
- Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a Flag tag at the C-terminus.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Loaded Human PD-1-Fc(PKSH033554) on Protein A Biosensor, can bind Human PD-L1-Flag(PKSH032870) with an affinity constant of 0.19 uM as determined in BLI assay.
-
-
CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His-Avi Tag)
PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) protein (His tag,mFc Tag)PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag)PD-L1 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (mFc Tag)PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His tag)PD-L1 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (hFc Tag)PD-L1 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His tag)PD-L1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag,AVI tag,Biotin)PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD274 (PD-L1) (AA 18-238) protein (His-DYKDDDDK-Strep II Tag)PD-L1 宿主: 人 宿主: HEK-293T Cells Recombinant ELISA, PI, SDS, WB
CD274 (PD-L1) (AA 19-238) (Active) protein (Fc Tag)PD-L1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- PD-L1 (CD274 (PD-L1))
-
别名
- PD-L1/B7-H1/CD274
-
背景
- B7 Homolog 1,B7-H1,B7H1,CD274,PD-L1,PDCD1 Ligand 1,PDCD1L1,PDCD1LG1,PDL1,Programmed Cell Death 1 Ligand 1,Programmed Death Ligand 1,CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
-
分子量
-
Calculated MW: 26.3 kDa
Observed MW: 35-40 kDa
-
基因ID
- 29126
-
UniProt
- Q9NZQ7
-
途径
- Cancer Immune Checkpoints
抗原
-
