CD2 Protein (CD2) (His tag)
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Quick Overview for CD2 Protein (CD2) (His tag) (ABIN7194720)
抗原
See all CD2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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标记
- This CD2 protein is labelled with His tag.
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原理
- Recombinant Human CD2 Protein (His Tag)
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序列
- Met 1-Asp 209
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产品特性
- A DNA sequence encoding the human CD2 (NP_001758.2) extracellular domain (Met 1-Asp 209) was expressed, fused with a polyhistidine tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized human CD2-His at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.1 μg/ml.
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CD2 (CD2) (AA 25-209) protein (Fc Tag)
CD2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
CD2 (CD2) (AA 1-351) protein (GST tag)CD2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
CD2 (CD2) (AA 25-209) protein (His-IF2DI Tag)CD2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
CD2 (CD2) (AA 25-209) protein (His tag)CD2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % SDS
CD2 (CD2) (AA 25-209) protein (His tag)CD2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
CD2 (CD2) proteinCD2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % pure SDS
CD2 (CD2) protein (His tag)CD2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by reducing SDS-PAGE.
CD2 (CD2) (AA 1-202) protein (Fc Tag)CD2 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
CD2 (CD2) (AA 25-209) (Active) protein (His tag,AVI tag,Biotin)CD2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD2 (CD2) (AA 25-209) (Active) protein (His tag)CD2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD2
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别名
- CD2
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背景
- Erythrocyte receptor,LFA-2,LFA-3 receptor,Rosette receptor,SRBC,T-cell surface antigen CD2,T-cell surface antigen T11/Leu-5,T11,T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.
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分子量
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Calculated MW: 22.7 kDa
Observed MW: 37 kDa
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基因ID
- 914
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NCBI登录号
- NP_001758
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UniProt
- P06729
抗原
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