Cathepsin L Protein (His tag)
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Quick Overview for Cathepsin L Protein (His tag) (ABIN7194682)
抗原
See all Cathepsin L (CTSL1) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Cathepsin L protein is labelled with His tag.
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原理
- Recombinant Human Cathepsin L/CTSL Protein (His Tag)
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序列
- Met 1-Val 333
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产品特性
- A DNA sequence encoding the pro form of human Cathepsin-L1 (NP_001903.1) (Met 1-Val 333) was expressed, fused with a polyhistidine tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Cathepsin L1 (CTSL1) (AA 1-333) protein (GST tag)
CTSL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin L1 (CTSL1) (AA 18-333) protein (GST tag)CTSL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin L1 (CTSL1) (AA 18-333) protein (His-IF2DI Tag)CTSL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cathepsin L1 (CTSL1) (AA 18-334) protein (His tag)CTSL1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Cathepsin L1 (CTSL1) (AA 1-334) protein (His tag)CTSL1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM NaAc, 0.1M NaCl, pH 5.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
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别名
- Cathepsin L/CTSL
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背景
- CTSL,CTSL1,Cathepsin L1,MEP,Major Excreted Protein,Cathepsin L is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism, and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. Like many proteases, Cathepsin L is synthesized as an inactive preproenzyme, and cleavage of the 96-residue proregion is necessary to generate the fully active 221-residue mature enzyme. Studies have demonstrated that cleavage of the proregion occur autocatalytically under acidic conditions. The enzyme takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin and native collagen and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Recently, Cathepsin L has been shown to suppress Th1 immune response in infected laboratory animals making them susceptible to concurrent bacterial infections. Cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells, and induced by growth factors and tumor promoters. In addition to its role in protein degradation, evidence has accumulated for the participation of Cathepsin L in various physiological and pathological processes, such as tumor invasion and metastasis, bone resorption, spermatogenesis, and arthritis. Accordingly, Cathepsin L may prove useful as a diagnostic or prognostic marker of human tumor malignancy.
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分子量
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Calculated MW: 37.3 kDa
Observed MW: 37 kDa
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基因ID
- 1514
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NCBI登录号
- NP_001903
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UniProt
- P07711
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途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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