CTNNB1 Protein (His-GST)
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Quick Overview for CTNNB1 Protein (His-GST) (ABIN7317597)
抗原
See all CTNNB1 蛋白蛋白类型
宿主
资源
纯度
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标记
- This CTNNB1 protein is labelled with His-GST.
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原理
- Recombinant Human Beta-Catenin/CTNNB1 Protein (His & GST Tag)
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序列
- Met 1-Leu 781
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产品特性
- A DNA sequence encoding the human CTNNB1 (P35222-1) (Met 1-Leu 781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 1-781) protein (GST tag)
CTNNB1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 682-781) protein (GST tag)CTNNB1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 1-781) protein (His-GST)CTNNB1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 2-781), (partial) protein (GST tag)CTNNB1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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别名
- Beta-Catenin/CTNNB1
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背景
- CTNNB,MRD19,armadillo,beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of CTNNB1 is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
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分子量
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Calculated MW: 113 kDa
Observed MW: 116 kDa
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基因ID
- 1499
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UniProt
- P35222
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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