CSNK1G1 Protein (His-GST)
This Recombinant CSNK1G1 protein is expressed in Baculovirus infected Insect Cells.
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产品编号 ABIN7317070
发货至:
中国
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Quick Overview for CSNK1G1 Protein (His-GST) (ABIN7317070)
抗原
See all CSNK1G1 蛋白
CSNK1G1
(Casein Kinase 1, gamma 1 (CSNK1G1))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
Baculovirus infected Insect Cells
纯度
> 94 % as determined by reducing SDS-PAGE.
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标记
- This CSNK1G1 protein is labelled with His-GST.
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原理
- Recombinant Human CSNK1G1/CKI-gamma 1 Protein (His & GST Tag)
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序列
- Met 1-Lys 422
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产品特性
- A DNA sequence encoding full length of human CSNK1G1 (NP_071331.2) (Met 1-Lys 422) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 4 nmol/min/mg using casein as substrate.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))
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别名
- CSNK1G1/CKI-gamma 1
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背景
- CK1gamma1,Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Thecasein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators ofsignal transductionpathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine-threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53 % -98 % identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates.
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分子量
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Calculated MW: 76.3 kDa
Observed MW: 66 kDa
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基因ID
- 53944
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NCBI登录号
- NP_071331
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UniProt
- Q9HCP0
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途径
- Hedgehog Signaling
抗原
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