Quick Overview for FGF2 蛋白 (ABIN6700921)
抗原
See all FGF2 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
-
-
Supplier Product No.
- 009-f01-u84-0010
-
Supplier
- Rockland
-
原理
- Human Fibroblast Growth Factor 154 basic Recombinant Protein (Animal Free)
-
纯化方法
- Fibroblast Growth Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.
-
内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
-
Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1 ng/mL.
-
-
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) protein
FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Fibroblast Growth Factor 2 (Basic) (FGF2) (full length) (Active) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE CC Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 135-288), (Isoform 1) (Active) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (AVI tag,His tag,Biotin)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 9-154) protein (His tag)FGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) protein (GST tag)FGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
-
-
应用备注
-
Application Note: Fibroblast Growth Factor 154 basic Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor 154 basic in immunological assays. Lyophilized from 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
-
缓冲液
- Lyophilized from 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.
-
储存液
- Without preservative
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
-
有效期
- 6 months
-
-
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
-
别名
- FGF2
-
背景
-
Synonyms: Heparin-binding growth factor 2 (HBGF-2), Prostatropin, Basic fibroblast growth factor (bFGF)
Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 154, also known as FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 154 (FGF-2) is a non-glycosylated protein, containing 154 amino acids, with a molecular weight of 17.2 kDa.
-
UniProt
- P09038
-
途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
抗原
-
