Quick Overview for CCL4 蛋白 (ABIN6700901)
抗原
See all CCL4 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-b85
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Supplier
- Rockland
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原理
- MIP-1ß Mouse Recombinant Protein
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纯化方法
- Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non- reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
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Biological Activity Comment
- Mouse Macrophage Inflammatory Protein-1 beta is fully biologically active when compared to standard. The activity is calculated by the ability to chemoattract blood monocytes at 20-100 ng/mL.
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Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) (Active) protein
CCL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant CMA Active
Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) (Active) protein (Biotin)CCL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA Active
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应用备注
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Application Note: MIP-1ß protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-1ß in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
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别名
- Ccl4
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背景
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Synonyms: Mouse Macrophage Inflammatory Protein-1-beta (CCL4), C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, Immune activation protein 2, ACT-2, ACT2, Protein H400, SIS-gamma, Mip1b, Scya4
Background: MIP-1ß is a secreted monokine with inflammatory and chemokinetic properties and can exist as a homodimer. It belongs to the intercrine beta (chemokine CC) family. Recombinant Mouse Macrophage Inflammatory Protein-1 beta produced in E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7,808 Daltons.
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UniProt
- P14097
抗原
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