14-3-3 theta Protein (YWHAQ) (GST tag)
Quick Overview for 14-3-3 theta Protein (YWHAQ) (GST tag) (ABIN6700782)
抗原
See all 14-3-3 theta (YWHAQ) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This 14-3-3 theta protein is labelled with GST tag.
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Supplier Product No.
- 009-001-r29s
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Supplier
- Rockland
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原理
- 14-3-3 theta recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length human 14-3-3θ was expressed in E. coli cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
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14-3-3 theta (YWHAQ) (AA 1-245) protein (His tag)
custom-made YWHAQ 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 theta (YWHAQ) (AA 1-245) protein (His tag)custom-made YWHAQ 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 theta (YWHAQ) (AA 1-245) protein (His tag)YWHAQ 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
14-3-3 theta (YWHAQ) (AA 1-245) protein (GST tag)YWHAQ 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
14-3-3 theta (YWHAQ) (AA 1-245) proteinYWHAQ 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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应用备注
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Application Note: 14-3-3θ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3θ Protein is suitable for use in Western Blot. Expect a band approximately ~ 56 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- 14-3-3θ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- 14-3-3 theta (YWHAQ)
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别名
- YWHAQ
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背景
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Synonyms: 14-3-3 theta, YWHAQ, 1C5, HS1, 14-3-3, 14-3-3 protein tau
Background: 14-3-3θ (also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3θ plays an important role in controlling apoptosis (1). Induced expression of 14-3-3θ protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3θ has been observed to mediate nucleocytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2). 14-3-3θ Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.
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NCBI登录号
- NM_006142
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途径
- Apoptosis, Myometrial Relaxation and Contraction
抗原
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