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14-3-3 theta Protein (YWHAQ)

This Recombinant 14-3-3 theta protein is expressed in 大肠杆菌(E. Coli).
Rockland
产品编号 ABIN6700780
Supplier Product No.: 009-001-r30s
发货至: 中国
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Quick Overview for 14-3-3 theta Protein (YWHAQ) (ABIN6700780)

抗原

See all 14-3-3 theta (YWHAQ) 蛋白
14-3-3 theta (YWHAQ)

蛋白类型

Recombinant

宿主

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资源

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大肠杆菌(E. Coli)

应用范围

Western Blotting (WB)

纯度

>90%
  • Supplier Product No.

    009-001-r30s

    Supplier

    Rockland

    原理

    14-3-3 theta recombinant protein

    纯化方法

    Recombinant full-length human 14-3-3θ was expressed in E. coli cells. The purity was determined to be >90% by densitometry.
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  • 应用备注

    Application Note: 14-3-3θ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3θ Protein is suitable for use in Western Blot. Expect a band approximately ~ 29 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 μg/μL

    缓冲液

    14-3-3θ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

    储存条件

    -80 °C

    储存方法

    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

    有效期

    12 months
  • 抗原

    14-3-3 theta (YWHAQ)

    别名

    YWHAQ

    背景

    Synonyms: 14-3-3 theta, YWHAQ, 1C5, HS1, 14-3-3, 14-3-3 protein tau

    Background: 14-3-3θ (also known as tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3θ plays an important role in controlling apoptosis (1). Induced expression of 14-3-3θ protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3θ has been observed to mediate nucleocytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2). 14-3-3θ Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.

    NCBI登录号

    NM_006142

    途径

    Apoptosis, Myometrial Relaxation and Contraction
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