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Quick Overview for TRAIL 蛋白 (ABIN6700756)
抗原
See all TRAIL (TNFSF10) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-w34-0010
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Supplier
- Rockland
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原理
- Human TRAIL Recombinant Protein
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纯化方法
- TRAIL purity was determined to be greater than 90% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensitive RPMI-8226 cells and is typically 10-15 ng/mL.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 118-281), (Trimer) protein (His-DYKDDDDK Tag)
TNFSF10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-281) protein (GST tag)TNFSF10 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 114-281) protein (His-DYKDDDDK-Strep II Tag)TNFSF10 宿主: 人 宿主: HEK-293T Cells Recombinant ELISA, PI, SDS, WB
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 118-291) protein (His tag)TNFSF10 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) protein (His-IF2DI Tag)TNFSF10 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 114-281) proteinTNFSF10 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 114-281) protein (mFc Tag)TNFSF10 宿主: 人 宿主: Mammalian Cells Recombinant Greater than 95 % as determined by SDS-PAGE.
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应用备注
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Application Note: TRAIL Recombinant Protein ha been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-TRAIL in immunological assays. Product is provided as lyophilized powder in 0.05M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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缓冲液
- Product is provided as lyophilized powder in 0.05M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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别名
- TNFSF10
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背景
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Synonyms: Apo2 Ligand (Apo2L), TL2, TNF-related apoptosis-inducing ligand, CD253
Background: TNF-Related Apoptosis-Inducing Ligand (TRAIL) is produced by a wide variety of cell types and is shown to be a cytotoxic protein that induces apoptosis in tumor cells through activation of the death receptors, DR4 and DR5. Human TRAIL is active on mouse cells. Recombinant human TRAIL is a non-glycosylated protein, containing the 168 amino acid extracellular portion of TRAIL that is homologous to TNF. It has a molecular weight of 19.5 kDa.
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UniProt
- P50591
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途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
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