TAF1 Protein (N-Term) (GST tag)
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Quick Overview for TAF1 Protein (N-Term) (GST tag) (ABIN6700605)
抗原
See all TAF1 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- N-Term
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标记
- This TAF1 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-t71s
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Supplier
- Rockland
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原理
- TAF1 (N-term) recombinant protein-GST fusion protein
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纯化方法
- Recombinant human TAF1 (1-561) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
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TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1400-1518) protein (His-IF2DI Tag)
TAF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1519-1651) protein (His-IF2DI Tag)TAF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1400-1651) protein (His-IF2DI Tag)TAF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1522-1656) protein (His tag,DYKDDDDK Tag)TAF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Bind, ScA
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应用备注
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Application Note: TAF1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. TAF1 Protein is suitable for use in Western Blot. Expect a band approximately ~130 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 μg/μL
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缓冲液
- TAF1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))
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别名
- TAF1
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背景
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Synonyms: BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, N-TAF1, NSCL2, OF, P250, TAF2A, Transcription initiation factor TFIID subunit 1
Background: TAF1 is a DNA-binding protein complex required for RNA polymerase II-mediated transcription of many, if not all, protein-encoding genes in eukaryotic cells. TAF1 plays a key role in the initiation process, since it binds to the TATA element to form a complex that nucleates the assembly of the other components into a preinitiation complex and that may be stable through multiple rounds of transcription. TAF1 may be targeted to specific chromatin-bound promoters and may play a key role in chromatin recognition (1). TAF1 also serves to link the control of transcription to the cell cycle (2). TAF1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cell Cycle, and Invasion/Metastasis research.
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NCBI登录号
- NM_138923
抗原
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