14-3-3 sigma/SFN Protein (GST tag)
Quick Overview for 14-3-3 sigma/SFN Protein (GST tag) (ABIN6700573)
抗原
See all 14-3-3 sigma/SFN (SFN) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This 14-3-3 sigma/SFN protein is labelled with GST tag.
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Supplier Product No.
- 009-001-r31s
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Supplier
- Rockland
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原理
- 14-3-3 sigma recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length human 14-3-3σ was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
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Stratifin (SFN) (AA 1-248) protein (His tag)
custom-made SFN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stratifin (SFN) (AA 1-248) protein (His tag)custom-made SFN 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stratifin (SFN) (AA 1-248) protein (GST tag)SFN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Stratifin (SFN) (AA 1-248), (full length) protein (GST tag)SFN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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应用备注
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Application Note: 14-3-3σ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3σ Protein is suitable for use in Western Blot. Expect a band approximately ~ 51 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- 14-3-3σ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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别名
- YWHAS
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背景
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Synonyms: 14-3-3 sigma, SFN, stratifin, YWHAS
Background: 14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ -deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death. 14-3-3σ Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.
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NCBI登录号
- NM_006826
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途径
- p53 Pathway, Myometrial Relaxation and Contraction
抗原
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