Stathmin 1 Protein (STMN1) (His tag)
Quick Overview for Stathmin 1 Protein (STMN1) (His tag) (ABIN6700567)
抗原
See all Stathmin 1 (STMN1) 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
标记
- This Stathmin 1 protein is labelled with His tag.
-
Supplier Product No.
- 009-001-t58s
-
Supplier
- Rockland
-
原理
- Stathmin recombinant protein-HIS Epitope
-
纯化方法
- Recombinant full-length human Stathmin protein was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >90% by densitometry.
-
-
Stathmin 1 (STMN1) (AA 1-149) protein (His tag)
STMN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Stathmin 1 (STMN1) (AA 64-149) protein (His-IF2DI Tag)STMN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Stathmin 1 (STMN1) (AA 1-149) protein (GST tag)STMN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Stathmin 1 (STMN1) (Transcript Variant 4) protein (His tag)STMN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Stathmin 1 (STMN1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)STMN1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Stathmin 1 (STMN1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)STMN1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
应用备注
-
Application Note: Stathmin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. Stathmin Protein is suitable for use in Western Blot. Expect a band approximately ~19 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 μg/μL
-
缓冲液
- Stathmin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
-
储存条件
- -80 °C
-
储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
-
有效期
- 12 months
-
-
- Stathmin 1 (STMN1)
-
别名
- STMN1
-
背景
-
Synonyms: STMN1, C1orf215, FLJ32206, Lag, LAP18, MGC138869, MGC138870, OP18, PP17, PP19, PR22, SMN, Stathmin
Background: Stathmin is a ubiquitous, highly conserved protein present in the cytoplasm in a variety of unphosphorylated and phosphorylated forms. Stathmin functions as an intracellular relay integrating regulatory signals of the cellular environment (1). Stathmin is involved in the regulation of the microtubule filament system. Stathmin binds tubulin in a ternary complex, with 2 tubulins for every stathmin and this complex interferes with microtubule dynamics in vitro and in vivo. Overexpression of several stathmin mutants suggest that stathmin induces depolymerization of interphase and mitotic microtubules in its unphosphorylated state, but is inactivated by phosphorylation in mitosis (2). Stathmin Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cancer, Invasion/Metastasis, and Neurobiology research.
-
途径
- MAPK Pathway, Microtubule Dynamics
抗原
-
