SIRT3 Protein (GST tag)
Quick Overview for SIRT3 Protein (GST tag) (ABIN6700531)
抗原
See all SIRT3 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This SIRT3 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-t33s
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Supplier
- Rockland
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原理
- SIRT3 recombinant protein-GST fusion protein
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纯化方法
- Full-length recombinant human SIRT3 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.
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Sirtuin 3 (SIRT3) (AA 1-399) protein (GST tag)
SIRT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 3 (SIRT3) (AA 118-399) protein (His tag)SIRT3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Sirtuin 3 (SIRT3) (AA 297-399) protein (GST tag)SIRT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 3 (SIRT3) (AA 5-257) (Active) protein (His tag)SIRT3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 90 % AcA, CC Active
Sirtuin 3 (SIRT3) (AA 127-303) (Active) protein (His tag)SIRT3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
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应用备注
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Application Note: SIRT3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT3 Protein is suitable for use in Western Blot. Expect a band approximately ~66 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- SIRT3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- SIRT3 (Sirtuin 3 (SIRT3))
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别名
- SIRT3
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背景
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Synonyms: SIR2L3, Sirtuin 3,NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, SIRT3, SIR2L3
Background: SIRT3 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT3 being a member of class I. SIRT3 contains an N-terminal mitochondrial targeting signal and a central catalytic domain and localizes in the nuclei and mitochondria (1). SIRT3 has NAD(+)-dependent histone deacetylase activity with specificity for lys16 of H4 and, to a lesser extent, lys9 of H3. SIRT3 can repress transcription of a target gene in vivo when recruited to its promoter. Full-length SIRT3 is transported from the nucleus to the mitochondria during cell stress conditions (2). SIRT3 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.
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NCBI登录号
- NM_012239
抗原
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