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SCYL1 Protein (GST tag)

This Recombinant SCYL1 protein is expressed in Insect cells (Sf9).
Rockland
产品编号 ABIN6700489
Supplier Product No.: 009-001-t26s
发货至: 中国
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Quick Overview for SCYL1 Protein (GST tag) (ABIN6700489)

抗原

See all SCYL1 蛋白
SCYL1 (SCY1-Like 1 (SCYL1))

蛋白类型

Recombinant

宿主

  • 3
  • 2

资源

  • 3
  • 2
Insect cells (Sf9)

应用范围

Western Blotting (WB)

纯度

>95%
  • 标记

    This SCYL1 protein is labelled with GST tag.

    Supplier Product No.

    009-001-t26s

    Supplier

    Rockland

    原理

    SCYL1 recombinant protein-GST fusion protein

    纯化方法

    Recombinant human SCYL1 (1-556) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
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  • 应用备注

    Application Note: SCYL1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SCYL1 Protein is suitable for use in Western Blot. Expect a band approximately ~85 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 μg/μL

    缓冲液

    SCYL1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

    储存条件

    -80 °C

    储存方法

    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

    有效期

    12 months
  • 抗原

    SCYL1 (SCY1-Like 1 (SCYL1))

    别名

    SCYL1

    背景

    Synonyms: GKLP, HT019, NKTL, NTKL, P105, TAPK, TEIF, TRAP, N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase

    Background: SCYL1 is a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. SCYL1 has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. SCYL1 activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. SCYL1 forms multimers following transfection into COS-7 cells (1). A SCYL1 binding protein has been identified that co-localized with SCYL1 in cytoplasm and shows ubiquitous expression (2). SCYL1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Inflammation, Invasion/Metastasis, and Neurobiology research.

    NCBI登录号

    NM_020680

    途径

    p53 Pathway
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