Retinoblastoma 1 Protein (RB1) (GST tag)
Quick Overview for Retinoblastoma 1 Protein (RB1) (GST tag) (ABIN6700479)
抗原
See all Retinoblastoma 1 (RB1) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Retinoblastoma 1 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-t17s
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Supplier
- Rockland
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原理
- Rb recombinant protein-GST fusion protein
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纯化方法
- Recombinant human Rb (48 - 378) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
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Retinoblastoma 1 (RB1) (AA 371-470) protein (GST tag)
RB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Application Note: Rb Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. Rb Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~62 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
Other: Kinase Assay-User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- Rb Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- Retinoblastoma 1 (RB1)
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别名
- RB1
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背景
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Synonyms: RB1, OSRC, pRb, pp110, p105-Rb, Retinoblastoma 1
Background: Rb is the retinoblastoma gene product and was the first tumor suppressor cloned. Rb is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F and repress transcription of genes required for S phase (1). Rb can bind to MDM2 and this overcomes both the antiapoptotic function of MDM2 and the MDM2-dependent degradation of p53. Rb associates with the Polycomb group (PcG) proteins to form a repressor complex that blocks entry of cells into mitosis (2). Rb colocalizes with nuclear PcG complexes and is important for association of PcG complexes with nuclear targets. Rb Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Cancer, Cell Cycle, and Neurobiology research.
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NCBI登录号
- NM_000321
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途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
抗原
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