PPP2CB Protein (GST tag)
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- 抗原 See all PPP2CB 蛋白
- PPP2CB (Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This PPP2CB protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-t01s
- Supplier
- Rockland
- 原理
- PP2A beta recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human PP2Aβ was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.
- 纯度
- >75%
- Top Product
- Discover our top product PPP2CB 蛋白
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Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB) (AA 1-309) protein (His tag)
custom-made PPP2CB 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB) (AA 1-309) protein (His tag)custom-made PPP2CB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB) (AA 1-309) protein (GST tag)PPP2CB 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB) (AA 1-309), (full length) protein (GST tag)PPP2CB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: PP2Aβ Protein is suitable for use in Western Blot. Expect a band approximately ~59 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 μg/μL
- 缓冲液
- PP2Aβ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- PPP2CB (Protein Phosphatase 2, Catalytic Subunit, beta Isozyme (PPP2CB))
- 别名
- PPP2CB (PPP2CB 产品)
- 背景
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Synonyms: PP2A-beta, PP2CB, PPP2CB, PPP2CB protein phosphatase 2
Background: PP2A is one of the four major Ser/Thr phosphatases which are implicated in the negative control of cell growth and division and encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A (1). PP2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B which is encoded by a diverse set of genes. The interaction was independent of ATP or magnesium ion and the inclusion of PTPA altered the substrate specificity of PP2A, with enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2). PP2Aβ Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Metabolic Disorder, Phosphatases, Ser/Thr Kinases, and WNT Signaling research.
- NCBI登录号
- NM_004156
- 途径
- Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades
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