KDM5B Protein (GST tag)
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北京 101111
Quick Overview for KDM5B Protein (GST tag) (ABIN6700243)
抗原
See all KDM5B 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This KDM5B protein is labelled with GST tag.
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Supplier Product No.
- 010-001-s36s
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Supplier
- Rockland
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原理
- KDM5B recombinant protein-GST fusion protein
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纯化方法
- Recombinant mouse KDM5B (1-753) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Mouse价格从 127,054.94 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 1-1544) protein (His tag)
custom-made KDM5B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 1-1544) protein (His tag)custom-made KDM5B 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysine (K)-Specific Demethylase 5B (KDM5B) (full length ) protein (DYKDDDDK Tag)KDM5B 宿主: 人 宿主: Insect cells (Sf9) Recombinant EAA, ScA
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应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: KDM5B Protein is suitable for use in Western Blot. Expect a band approximately ~112 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 μg/μL
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缓冲液
- KDM5B Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))
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别名
- Kdm5b
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背景
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Synonyms: Jarid1b,PUT1,Plu1,PLU-1,Rb-Bp2, AW556288, RBBP2H1A, mKIAA4034, Lysine-specific demethylase 5B, EC 1.14.11.-, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1
Background: KDM5B is a lysine (K)-specific demethylase 5B which is highly expressed in testis and shows the lowest expression in skeletal muscle (1). KDM5B is a histone H3 lysine 4 (H3K4) demethylase up-regulated in prostate cancer and associated with androgen receptor where it regulated its transcriptional activity in reporter gene assays (2). Overexpression of KDM5B in HeLa cells results in loss of tri-, di-, and monomethyl H3K4, but does not alter the methylation status of H3K9 or H3K36. A KDM5B containing highly conserved putative DNA/chromatin binding motif is specifically up-regulated in breast cancer. KDM5B Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.
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途径
- Warburg Effect
抗原
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