KDM5B Protein (full length) (DYKDDDDK Tag)
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- 抗原 See all KDM5B 蛋白
- KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This KDM5B protein is labelled with DYKDDDDK Tag.
- 应用范围
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- 产品特性
- Recombinant JARID1B / KDM5B (accession number NP_006609, full length) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 179.9 kDa.
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Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 231-319) protein (GST tag)
KDM5B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 1-1544) protein (Strep Tag)Crystallography grade KDM5B 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 1-1544) protein (Strep Tag)Crystallography grade KDM5B 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- 应用备注
- Recombinant JARID1B / KDM5B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Catalytic Rate: 20 turnovers/ enzyme molecule/ hour. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 50 nM Recombinant JARID1B / KDM5B protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- 限制
- 仅限研究用
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- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))
- 别名
- JARID1B / KDM5B (KDM5B 产品)
- 别名
- jarid1b Protein, jarid1ba Protein, id:ibd5050 Protein, wu:fd15c05 Protein, cb264 Protein, jarid1bb Protein, plu1 Protein, wu:fc44h11 Protein, zgc:85741 Protein, JARID1B Protein, CT31 Protein, PLU-1 Protein, PLU1 Protein, PUT1 Protein, RBBP2H1A Protein, 2010009J12Rik Protein, 2210016I17Rik Protein, AW556288 Protein, D1Ertd202e Protein, Jarid1b Protein, Plu1 Protein, Rb-Bp2 Protein, mKIAA4034 Protein, RGD1565602 Protein, lysine (K)-specific demethylase 5Ba Protein, lysine (K)-specific demethylase 5Bb Protein, lysine demethylase 5B Protein, lysine (K)-specific demethylase 5B Protein, kdm5ba Protein, kdm5bb Protein, KDM5B Protein, Kdm5b Protein
- 背景
- Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.
- 分子量
- 179.9 kDa
- 途径
- Warburg Effect
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