Quick Overview for IL-7 蛋白 (ABIN6700167)
抗原
See all IL-7 (IL7) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-b94
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Supplier
- Rockland
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原理
- IL-7 Human Recombinant Protein
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纯化方法
- Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.
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内毒素水平
- Measured by LAL is 0.01 EU/μg protein.
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Biological Activity Comment
- Recombinant H-IL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of mouse 2E8 cells is less then 0.5ng/ml.
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Interleukin 7 (IL7) (AA 26-154) (Active) protein (His tag)
IL7 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 7 (IL7) (AA 26-177) (Active) protein (His tag)IL7 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Interleukin 7 (IL7) (AA 26-177) protein (His-IF2DI Tag)IL7 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 7 (IL7) (AA 26-154) (Active) protein (His tag)IL7 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 7 (IL7) (Active) protein (His tag)IL7 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 7 (IL7) (AA 26-177) protein (GST tag)IL7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Application Note: IL-7 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-7 in immunological assays. Buffer Formulation: 10 mM Acetic Acid.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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浓度
- 0.1 mg/mL
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缓冲液
- Buffer Formulation: 10 mM Acetic Acid.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-7 (IL7) (Interleukin 7 (IL7))
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别名
- IL7
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背景
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Synonyms: Interleukin 7 cytokine, Interleukin-7, IL-7
Background: Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis because it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients. IL-7 may also support bioenergetics that control cell fate decisions in lymphocytes. Recombinant Human IL-7 produced in E. coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17,412 Daltons.
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UniProt
- D0EYG7
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途径
- JAK/STAT Signaling
抗原
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