FLT3LG 蛋白
Quick Overview for FLT3LG 蛋白 (ABIN6699875)
抗原
See all FLT3LG 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-u86-0010
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Supplier
- Rockland
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原理
- Mouse Flt-3 Ligand Recombinant Protein
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纯化方法
- Flt-3 Ligand purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse AML5 cells and is typically 5-8 ng/mL.
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应用备注
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Application Note: Flt-3 Ligand Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti- Flt-3 Ligand in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).
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: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).
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- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
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别名
- Flt3lg
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背景
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Synonyms: FLt3 L, Fms-related tyrosine kinase 3 ligand
Background: FMS-related Tyrosine Kinase 3 Ligand (FLT-3 Ligand) is a growth factor important for the proliferation of hematopoietic cells. FLT-3 Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). FLT-3 Ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human FLT-3 Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors. Recombinant mouse FLT-3 Ligand is a non-glycosylated protein, containing 163 amino acids, with a molecular weight of 18.6 kDa.
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UniProt
- P49772
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途径
- RTK signaling
抗原
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