Quick Overview for FGF21 蛋白 (ABIN6699850)
抗原
See all FGF21 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-u77-0005
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Supplier
- Rockland
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原理
- Human Fibroblast Growth Factor-21 Recombinant Protein
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纯化方法
- Fibroblast Growth Factor-21 purity was determined to be greater than 95% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of NIH 3T3 cells in the presence of 5 μg/mL FGF binding protein and is typically 0.12-0.6 μg/mL.
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Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (mFc-Avi Tag)
FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (His tag)FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (mFc-Avi Tag,Biotin)FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (His tag)FGF21 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-210) protein (His tag)FGF21 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (Active) proteinFGF21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 21 (FGF21) (AA 29-208) protein (His tag)FGF21 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (Fc Tag)FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fibroblast Growth Factor 21 (FGF21) (AA 30-209) protein (GST tag)FGF21 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Application Note: Fibroblast Growth Factor-21 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-21 in immunological assays. Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
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缓冲液
- Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- FGF21 (Fibroblast Growth Factor 21 (FGF21))
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别名
- FGF21
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背景
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Synonyms: FGFL
Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-21 is most closely related to FGF-19 and shows low binding to heparin and is highly diffusible. Recombinant human FGF-21 is a non-glycosylated protein, containing 182 amino acids, with a molecular weight of 19.5 kDa.
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UniProt
- Q9NSA1
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途径
- RTK signaling
抗原
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