CDKL2 Protein (GST tag)
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- 抗原 See all CDKL2 蛋白
- CDKL2 (Cyclin Dependent Kinase Like 2 (CDKL2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This CDKL2 protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r67s
- Supplier
- Rockland
- 原理
- CDKL2 recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human CDKL2 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
- 纯度
- >80%
- Top Product
- Discover our top product CDKL2 蛋白
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Cyclin Dependent Kinase Like 2 (CDKL2) (AA 1-568) protein (His tag)
custom-made CDKL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin Dependent Kinase Like 2 (CDKL2) (AA 1-493) protein (His tag)custom-made CDKL2 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin Dependent Kinase Like 2 (CDKL2) protein (His tag)CDKL2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 80 % as determined by reducing SDS-PAGE.
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- 应用备注
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Application Note: CDKL2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CDKL2 Protein is suitable for use in Western Blot. Expect a band approximately ~ 81 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 μg/μL
- 缓冲液
- CDKL2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- CDKL2 (Cyclin Dependent Kinase Like 2 (CDKL2))
- 别名
- CDKL2 (CDKL2 产品)
- 背景
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Synonyms: KKIAMRE, P56, Cyclin-dependent kinase-like 2, Protein kinase p56 KKIAMRE, Serine/threonine-protein kinase KKIAMRE
Background: CDKL2 or cyclin-dependent kinase-like 2 is a member of a large family of CDC2-related serine/threonine protein kinases that is postnatally expressed in various brain regions. The extremely high CDKL2 expression in brain regions suggests that it has a role in cognition and emotion. CDKL2 transcripts increase in the deep cerebellar nuclei of the rabbit after eyeblink conditioning, a model of associative learning and memory CDKL2, also known as p56 KKIAMRE, accumulates primarily in the cytoplasm with lower levels in the nucleus (1). CDKL2 or p56, and the related protein kinase had a similar pattern of mRNA expression except in the reproductive organs. CDKL2 is expressed in several human tissues, with the highest expression in testis and kidney (2). CDKL2 Protein is ideal for investigators involved in Signaling Protein, Cellular Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.
- NCBI登录号
- NM_003948
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