c-FOS Protein (GST tag)
Quick Overview for c-FOS Protein (GST tag) (ABIN6699563)
抗原
See all c-FOS (c-Fos) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This c-FOS protein is labelled with GST tag.
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Supplier Product No.
- 009-001-r95s
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Supplier
- Rockland
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原理
- FOS recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length human FOS was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
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c-Fos (c-Fos) (AA 1-380) protein (His tag)
custom-made c-Fos 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
c-Fos (c-Fos) (AA 1-380) protein (His tag)custom-made c-Fos 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
c-Fos (c-Fos) (AA 2-180) protein (His-IF2DI Tag)c-Fos 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
c-Fos (c-Fos) (AA 1-380) protein (GST tag)c-Fos 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
c-Fos (c-Fos) (full length ) protein (His tag)c-Fos 宿主: 人 宿主: Baculovirus Recombinant The purified recombinant protein is greater than 90 % homogeneous and contains no detectable protease, DNase and RNase activity. PI
c-Fos (c-Fos) protein (His tag)c-Fos 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: FOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. FOS Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~78 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
Other: Kinase Assay-User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- FOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- c-FOS (c-Fos) (c-Fos)
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别名
- FOS
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背景
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Synonyms: AP-1, c-FOS, Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
Background: FOS is a member of the FOS gene family that consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. FOS gene encodes a leucine zipper protein that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation (1). In some cases, expression of the FOS gene has also been associated with apoptotic cell death. FOS overexpression leads to decreased phosphorylation and dimerization of STAT1, which in turn downregulates p21 gene expression (2). This regulatory pathway may enhance the proliferation of lymphocytes in rheumatoid arthritis patients. FOS Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, and Neurobiology research.
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NCBI登录号
- NM_005252
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途径
- S100 Proteins
抗原
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