BDNF 蛋白
(1 reference)
Quick Overview for BDNF 蛋白 (ABIN6699554)
抗原
See all BDNF 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
Supplier Product No.
- 009-001-c27
-
Supplier
- Rockland
-
原理
- BDNF Human Recombinant Protein
-
纯化方法
- Purity was determined to be greater than 97% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE against a known standard. Endotoxin level as determined by kinetic LAL analysis: 0.05 EUs/μg protein.
-
内毒素水平
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
-
Biological Activity Comment
- Proliferation of a neuroblastoma cell line stably expressing TrkB (BR6) ED50 ≤ 40 ng/mL (≥ 2.5 x 10^4 units/mg).
-
-
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-255) protein (GST tag)
BDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) protein (GST tag)BDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Brain-Derived Neurotrophic Factor (BDNF) (AA 137-237), (partial) protein (GST tag)BDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
-
-
应用备注
-
Application Note: BDNF has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-BDNF in immunological assays.
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
-
浓度
- 0.1 mg/mL
-
缓冲液
-
Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
-
储存液
- Without preservative
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
-
有效期
- 6 months
-
-
-
: "Isolation and Identification of the High-Affinity DNA Aptamer Target to the Brain-Derived Neurotrophic Factor (BDNF)." in: ACS chemical neuroscience, Vol. 15, Issue 2, pp. 346-356, (2024) (PubMed).
-
: "Isolation and Identification of the High-Affinity DNA Aptamer Target to the Brain-Derived Neurotrophic Factor (BDNF)." in: ACS chemical neuroscience, Vol. 15, Issue 2, pp. 346-356, (2024) (PubMed).
-
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
-
别名
- BDNF
-
背景
-
Synonyms: Brain-derived neurotrophic factor, Abrineurin cytokine
Background: BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum, it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. Recombinant Human Brain-Derived Neurotrophic Factor produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Daltons.
-
UniProt
- P23560
-
途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
抗原
-
