Annexin V Protein (His tag)
Quick Overview for Annexin V Protein (His tag) (ABIN6699527)
抗原
See all Annexin V (ANXA5) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Annexin V protein is labelled with His tag.
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Supplier Product No.
- 009-001-r40s
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Supplier
- Rockland
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原理
- Annexin 5 recombinant protein-HIS Epitope
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纯化方法
- Recombinant full-length human Annexin 5 Protein was expressed by E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
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应用备注
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Application Note: Annexin 5 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25 % glycerol. Annexin 5 Protein is suitable for use in Western Blot. Expect a band approximately ~ 37 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- Annexin 5 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- Annexin V (ANXA5) (Annexin A5 (ANXA5))
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别名
- ANXA5
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背景
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Synonyms: ANXA5, PP4, ANX5, ENX2, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha
Background: Annexin 5 is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. In vitro studies have shown that Annexin 5 is a cellular protein which plays a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin (1). Through affinity assays Annexin 5 has been localized on cells that expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death (2). Annexin 5 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Apoptosis/Autophagy, Cancer, and Neurobiology research.
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NCBI登录号
- NM_001154
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途径
- Apoptosis
抗原
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