PRMT1 Protein (AA 1-353) (His-MBP)
Quick Overview for PRMT1 Protein (AA 1-353) (His-MBP) (ABIN666862)
抗原
See all PRMT1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-353
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标记
- This PRMT1 protein is labelled with His-MBP.
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序列
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MAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR -
产品特性
- Human PRMT1 isoform3, 1-353 aa, Human, His-MBP tagged, Recombinant, E.coli
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纯化方法
- > 90% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 10 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group on histone H4 per minute at 37C.
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Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-371) protein (His tag)
custom-made PRMT1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-361) protein (His tag)PRMT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-353) (Active) protein (His-MBP)PRMT1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, EAA Active
Protein Arginine Methyltransferase 1 (PRMT1) protein (DYKDDDDK Tag)PRMT1 宿主: 人 宿主: Baculovirus Recombinant EAA, ScA
Protein Arginine Methyltransferase 1 (PRMT1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PRMT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-248), (partial) protein (GST tag)PRMT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 40 mM Tris-HCl buffer ( pH 8.0) containing 100 mM NaCl, 4 mM MgCl₂, 2 mM DTT, 40 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))
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别名
- PRMT1
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背景
- Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Recombinant human PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 84.2 kDa (750aa)
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NCBI登录号
- NP_938074
抗原
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