Quick Overview for DnaK (AA 385-638) 蛋白 (ABIN666810)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 385-638
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产品特性
- Dnak(C-term, 385-638), E.coli, Recombinant, E.coli
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DnaK (AA 385-546) protein
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 508-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 1-638), (full length) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 1-384) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 1-638), (full length) protein宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
DnaK (AA 1-384), (N-Term) protein宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/ml (determined by Bradford assay)
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缓冲液
- Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5 mM DTT, 10%Glycerol
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储存条件
- 4 °C
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- DnaK
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背景
- DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. The protein coding region of the substrate binding domain of DNAK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus. Synonyms: Dnak (C-term, 385-638), Substrate binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp 70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,. NCBI no.: NP_414555
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分子量
- 27.7kDa (255aa), confirmed by MALDI-TOF.
抗原
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