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DnaK 蛋白

宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 95 % as determined by SDS-PAGE and RP- HPLC WB
产品编号 ABIN7328699
发货至: 中国
  • 抗原
    DnaK
    蛋白类型
    Recombinant
    宿主
    资源
    • 6
    大肠杆菌(E. Coli)
    应用范围
    Western Blotting (WB)
    原理
    Recombinant Human DnaKHsp70) produced in E. coli is a single, non- glycosylated polypeptide chain containing 661 amino acids fused to His-tag at the N-terminus with a total molecular weight of 72.2kDa.
    序列
    MGSSHHHHHH SSGLVPRGSH MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGD TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D
    产品特性
    Escherichia coli
    纯度
    > 95 % as determined by SDS-PAGE and RP- HPLC
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 状态
    Liquid
    溶解方式
    Dilute in PBS or medium that is identical to that used in the assay system.
    浓度
    Lot specific
    缓冲液
    Sterile-filtered colorless solution contains 20 mM Tris, pH 7.5, and 2 mM DTT having a protein concentration of 1 mg/mL. 
    储存条件
    4 °C,-20 °C
  • 抗原
    DnaK
    背景
    Hsp70 plays an important role in protein folding and in protecting cells from stress. In most species there are many proteins that belong to the Hsp70 family. Some are expressed only under stress conditions while others are present in cells under normal conditions and are not heat-inducible.,Hsp70
    分子量
    72.2 kDa
    UniProt
    P08107
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