Quick Overview for DnaK (AA 1-384) 蛋白 (ABIN666809)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-384
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序列
- MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG
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产品特性
- Dnak(N-term, 1-384), E.coli, Recombinant, E.coli)
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纯化方法
- > 95% by SDS-PAGE
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DnaK (AA 1-638), (full length) protein
宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
DnaK (AA 1-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
DnaK (AA 385-546) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
DnaK (AA 508-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
DnaK (AA 385-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
DnaK (AA 1-384), (N-Term) protein宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 25 mM Tris-HCl buffer ( pH 7.5) containing 100 mM NaCl, 5 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DnaK
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背景
- DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain (residues385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques.
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分子量
- 41.6 kDa (384aa)
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NCBI登录号
- NP_414555
抗原
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