Quick Overview for DnaK (AA 508-638) 蛋白 (ABIN666807)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 508-638
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序列
- MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK
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产品特性
- Dnak(508-638), E.coli, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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DnaK (AA 1-638), (full length) protein
宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
DnaK (AA 1-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
DnaK (AA 385-546) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
DnaK (AA 1-384) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
DnaK (AA 385-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
DnaK (AA 1-384), (N-Term) protein宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 25 mM Tris-HCl buffer ( pH 7.5) containing 100 mM NaCl, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DnaK
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背景
- DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak (residues 508-638) of the substrate binding domain is alpha-helical and appears to act as a lid covering the substrate binding cleft. DnaK (amino acid 508-638) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus
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分子量
- 14.6 kDa (132aa)
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NCBI登录号
- NP_414555
抗原
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