MYDGF Protein (AA 25-166) (His tag)
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Quick Overview for MYDGF Protein (AA 25-166) (His tag) (ABIN6388124)
抗原
See all MYDGF (D17Wsu104e) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 25-166
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标记
- This MYDGF protein is labelled with His tag.
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序列
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VSEPTTV PFDVRPGGVV HSFSQDVGPG NKFTCTFTYA SQGGTNEQWQ MSLGTSEDSQ HFTCTIWRPQ GKSYLYFTQF KAELRGAEIE YAMAYSKAAF ERESDVPLKS EEFEVTKTAV SHRPGAFKAE LSKLVIVAKA ARSEL -
纯化方法
- > 95% by SDS-PAGE
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DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) (AA 32-173) protein (His tag)
D17Wsu104e 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) protein (His tag)D17Wsu104e 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) (AA 33-173) protein (His tag)D17Wsu104e 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- MYDGF (D17Wsu104e) (DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e))
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别名
- SF20/MYDGF
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背景
- Mydgf, also known as Myeloid-derived growth factor, is a bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and repair after myocardial infarction. It stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Mydgf-deficient mice develop larger infarct scars and more severe contractile dysfunction compared to wild-type mice. Recombinant mouse Mydgf, fused to His-tag at N-termineus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 18.1 kDa (165aa) Confirmed by MALDI-TOF
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NCBI登录号
- NP_543027
抗原
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