EPOR Protein (AA 25-250) (His tag)
Quick Overview for EPOR Protein (AA 25-250) (His tag) (ABIN6387943)
抗原
蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 25-250
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标记
- This EPOR protein is labelled with His tag.
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序列
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APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDP
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤70ng/ml with Human Erythropoietin.
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Erythropoietin Receptor (EPOR) (AA 25-138) protein (His-IF2DI Tag)
EPOR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Erythropoietin Receptor (EPOR) (AA 1-508) protein (GST tag)EPOR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (AA 25-250) protein (His tag)EPOR 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
Erythropoietin Receptor (EPOR) (Active) protein (His tag)EPOR 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- EPOR (Erythropoietin Receptor (EPOR))
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别名
- Erythropoietin R/EPOR
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背景
- EPOR, also known as Erythropoietin receptor, mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, it dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. So it may also activate the LYN tyrosine kinase. Recombinant human EPOR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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分子量
- 25.6 kDa (232aa)
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NCBI登录号
- NP_000112
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UniProt
- P19235
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途径
- JAK/STAT Signaling
抗原
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