TPI1 Protein (AA 1-249) (His tag)
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- 抗原 See all TPI1 蛋白
- TPI1 (Triosephosphate Isomerase 1 (TPI1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-249
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This TPI1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.
- Top Product
- Discover our top product TPI1 蛋白
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Triosephosphate Isomerase 1 (TPI1) (AA 1-249) protein (GST tag)
TPI1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Triosephosphate Isomerase 1 (TPI1) (AA 1-249) protein (His tag)TPI1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Triosephosphate Isomerase 1 (TPI1) protein (Myc-DYKDDDDK Tag)TPI1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- TPI1 (Triosephosphate Isomerase 1 (TPI1))
- 别名
- TPI1 (TPI1 产品)
- 背景
- TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 28.8kDa (269aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_000356
- UniProt
- P60174
- 途径
- Cell RedoxHomeostasis
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