GAPDH Protein (AA 1-335)
Quick Overview for GAPDH Protein (AA 1-335) (ABIN6387883)
抗原
See all GAPDH 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-335
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序列
- MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 50unit/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-335) protein (His tag)
GAPDH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-333) protein (His tag)GAPDH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) protein (GST tag)GAPDH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) protein (GST tag)GAPDH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) proteinGAPDH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36- kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1, 3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 36 kDa (335aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_002037
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UniProt
- P04406
抗原
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